Hill reaction and heated chloroplast
WebThe shoots of 16-day-old spring wheat plants (Triticum aestivumL., cv. Albidum 29) were subjected to heat shock (HS) at 40, 41, or 43°C for 10 min. The activity of the Hill reaction in chloroplasts isolated immediately after HS was 83, 61, and 30% of the initial value, respectively. The activity of the Hill reaction was also estimated after plant return to the … Web30 minutes. The ability of chloroplasts to catalyze the Hill reaction was most sensitive to heating at 51? C, but both treatments showed preferential de-struction of activity in the Hill reaction. The lability of the system responsible for the Hill reaction at high pH values agrees with data reported by Punnett (8).
Hill reaction and heated chloroplast
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WebPlace a test tube rack 25 cm from the light source with a “heat-sink” in between. Since the rate of the Hill reaction is affected by temperature, a container of water will be placed …
WebMar 1, 1979 · Inhibition of the photosynthetic CO 2-uptake in ivy leaves (Hedera helix L.) after heat stress is due to an impairment of the photosynthetic processes in the chloroplasts (BAUER, 1978). For a more definite evaluation of the site of heat damage, the activity of ribulose bisphosphate carboxylase (EC 4.1.1.39), the Hill reaction, and the ultrastructure … WebMay 2, 2024 · The Hill Reaction depends on electrons released during the light-dependent stage of photosynthesis being picked up by the blue electron acceptor DCPIP. The reaction can only occur if the thylakoid membranes are illuminated as the light-dependent stage stops in the dark. DCPIP is blue when oxidised (at pH 7.0) and colourless when reduced, …
WebThis is now known as the Hill reaction. You can examine a drop of the sediment extract with a microscope under high power to see chloroplasts. There will be fewer chloroplasts in the supernatant – which decolourises the DCPIP more slowly, reinforcing the idea that the reduction is the result of chloroplast activity. Sample results WebApr 6, 1971 · The inhibitory effect of carbonyl cyanide m-chlorophenylhydrazone (CCCP) on the electron transport system of chloroplasts was investigated.At concentrations higher than 1 · 10 −7 M, CCCP acted as an inhibitor of the Hill reaction. Two different processes of CCCP inhibition were discernible; the one which was accomplished very rapidly and …
WebBiology questions and answers. LAB #5: THE HILL REACTION IN ISOLATED CHLOROPLASTS INTRODUCTION Photosynthesis is the process by which plants convert light energy to chemical energy. The familiar equation summarizes the process but gives little indication of its complexity. The reaction sequences involve 2 stages which have …
Webthe chloroplasts stored in 10% glycerol at -23 C was 23 days. The ammonium ion at 0.01 M enhanced Hill reaction activity up to 171%. Leaves containing chloroplasts with the highest Hill reaction activity were found near the 8th node below the apex. Leaf water potentials less than -28 bars reduced the activity about 50%. Daylight conditions during phil hurley obituaryWebApr 2, 2024 · Extensive research over the past several decades has revealed that chloroplasts, the photosynthetic organelles of plants, are highly sensitive to heat stress, which affects a variety of photosynthetic processes including chlorophyll biosynthesis, photochemical reactions, electron transport, and CO 2 assimilation. phil hussey ibmWebHill Reaction Lab Report. Light Absorbance of Chloroplasts in Spinach and Romaine Lettuce Introduction: In this experiment, the purpose was to examine the light absorbance of … phil huss cpaWebSince the intact chloroplast does not contain catalase, H 2 O 2 being instead reduced by ascorbate, when Fd is oxidized in vivo by O 2 then one molecule of O 2 will be consumed … phil husseyhttp://people.hsc.edu/faculty-staff/edevlin/edsweb01/courses/cellbiology/labmanual/new_page_4.htm phil hurstWebHill Reaction Lab Report. Light Absorbance of Chloroplasts in Spinach and Romaine Lettuce Introduction: In this experiment, the purpose was to examine the light absorbance of solutions that contained chloroplasts, water, reaction buffer, DCPIP, and one with herbicide in addition. In order to do this, we used a spectrometer. phil husbandsWebNational Center for Biotechnology Information phil hurst blackpool tower