site stats

Phis2.1 vector

WebbA yeast one-hybrid library was constructed to screen the binding proteins of suspensor specific cis-element. In this study, five repeats of suspensor specific cis-element … Webb27 apr. 2024 · As with the positive control, yeast cells co-transformed with AD-PpPIF8 and proPpCHS-pHIS2.1 vectors could also grow on both selective mediums. However, the negative control yeast cells co-transformed with empty AD and proPpCHS -pHIS2.1 vectors could only grow on SD/−Leu−Trp medium ( Figure 11 F).

Identification and characterization of the novel Col10a1 regulatory ...

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html WebbpTALEN v2 (NN) pTALEN v2 (HD) pX335 pX260 pX320 pShuttle-CMV pShuttle pAdTrack-CMV pAdTrack pAdEasy-1 BJ5183 AdEasy-1 pAd/BLOCK-iT-DEST RNAi Gateway Vector pDC315 pBHGloxdelE13cre pShuttle-IRES-hrGFP2 pAAV-MCS pAAV-RC2 pAAV-RC pHelper pAAV-LacZ pAAV-IRES-hrGFP pCMV-MCS RNAi-Ready pSIREN-RetroQ RNAi-Ready … how to set up a refractor telescope https://sofiaxiv.com

petunia transcription factor, PhOBF1, regulates flower senescence …

WebbChina Agricultural University (CAU, Chinese: 中国农业大学; pinyin: Zhōngguó Nóngyè Dàxué; abbreviated 农大/Nóngdà) is a university in Beijing, People's Republic of China specializing in agriculture, biology, engineering, veterinary medicine, economics, management, humanities and social science. Webb8 juli 2024 · pHis2 编号 载体名称 北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息 出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体 高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶 载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签: -- 载体抗性: 卡纳霉素 筛选标记: -- 备注: 含HIS3 报告基因 产品目录号: -- … WebbClose. We will increase some of our prices on April 1, 2024. Be sure to complete your order before this time to take advantage of current prices. See the new prices and get more … notewriter in epic

酵母单杂交详细实验流程-钟鼎生物

Category:pHIS2 - honorgene.com

Tags:Phis2.1 vector

Phis2.1 vector

Genes Free Full-Text Heterologous Expression of GbTCP4, a …

Webb24 jan. 2024 · For ligation: the molar ratio of your bait sequence: vector ranges from 3:1 and 5:1. The reaction can be put at 16 degrees overnight (in PCR cycler machine). It … WebbNo. PT3024-1). The full sequence of differential sequence of LRK6 promoter 2 (DSLP2) (5k-CTC-TAAAGTTAAGT-3k) from the rice LRK6 promoter was synthesized into three tandemly repeated copies and then inserted into the EcoRI–MluI sites of the multiple cloning site (MCS) upstream of the HIS3 minimal promoter in the pHIS2.1 expression …

Phis2.1 vector

Did you know?

Webb27 mars 2024 · 筛选含有报道质粒的酵母细胞 具体过程为： 合成靶序列； 将靶序列插入pHIS2载体的多克隆位点； 一般为将0.1mg pHIS2质粒用EcoRⅠ和SacⅠ进行完全双酶 …

WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 Webb29 okt. 2024 · DNA-protein interactions were verified by growth performance of yeast cells co-transformed with the indicated pairs of Rec2 and pHis2.1 vectors on synthetic defined (SD) medium –Leu–Trp–His containing 3-amino-1,2,4-triazole (3-AT).

WebbUnique restriction sites are in bold. pHIS2.1 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the Matchmaker™ One-Hybrid Library Construction & Screening Kit (Cat. No. 630304). http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627

http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf

http://www.coolaber.com/usr/2160/File/F/F4671.pdf notf 02/2017Webb方法 通过克隆 MaAQP1 的启动子，将启动子构建到pHIS2诱饵质粒上，转化酵母菌构建诱饵表达载体，同时构建干旱胁迫的香蕉（ Musa acuminat L. AAA group cv. Brazilian）cDNA文库。 结果 克隆获得1 362 bp的启动子序列，通过分析其顺式作用元件，结果显示启动子序列中共有72个顺式作用元件，包括了TATA-box和CAAT-box核心元件，ABA响应元件 … how to set up a refurbished computerWebb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in … notex powderWebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. … notewritersWebb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which … noteys worldWebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … notezilla troubleshooterWebbVectors in Use. N or C-terminal His tag. Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. Parallel vector 2 contains N and C-terminal His (pET22), … how to set up a refurbished iphone se