Phis8质粒
Webb25 apr. 2024 · 质粒 (英语: Plasmid ),又名 质体 ,是指在细胞的 染色体 或核区 DNA 之外,能够自主复制的DNA分子(字源: plasm 为生殖质, -id 表示粒)。 质粒与染色体 … http://www.googbio.com/product/425385.html
Phis8质粒
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Webb篇二 :实验一、质粒DNA的提取及检测实验报告. 实验一、质粒DNA的提取及检测. 【实验目的】. 1、掌握碱裂解法提取质粒的原理和步骤. 2、掌握琼脂糖凝胶电泳检测DNA的方法 … Webbpet系列载体是目前应用最广泛的重组蛋白表达载体,载体构建时目的片段被克隆入载体噬菌体t7转录翻译系统下,经转化在宿主t7rna聚合酶的诱导下进行表达。t7rna聚合酶具有超 …
Webb17 feb. 2024 · 为了解决这个问题,Kleeman等人克隆并表征了13种单体荧光蛋白的检测灵敏度、光谱重叠和spillover,以确定在多色方案中的实用性。. 13种荧光蛋白的激发、发 … Webbphis8-3 (Search Vector Database) Backbone size w/o insert (bp) 5206 Total vector size (bp) 6204 Vector type Bacterial Expression Growth in Bacteria Bacterial Resistance (s) …
Webbversity Sequencing Facility, St. Louis, MO). The pHIS8-AtGCL expres-sion vector was constructed by digesting pGEM-T-AtGCL with NcoI and NotI and then ligating the fragment into a NcoI/NotI-digested pHIS8 vector (23). An expression construct of AtGCL with a truncated N terminus (pHIS8-AtGCL85) was generated by PCR using the ap-propriate ... WebbAnalyze Sequence: pHIS8. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about your order, deposit, or a plasmid?
Webb载体描述. pHISi is a yeast integration and reporter vector for use with the MATCHMAKER One-Hybrid System. pHISi contains the yeast HIS3 gene downstream of the MCS and the minimal promoter of the HIS3 locus (PminHIS). Cis-acting sequences of interest (i.e., target elements) can be inserted into the MCS.
Webbmiaolingbio.com greenwich power station jettyfoam cover for pipesWebb 使用QIAprep Spin Miniprep Kit纯化的pBluescript DNA的限制性酶切如图所示。使用图示的酶(1–5个单位)对1 µg质粒DNA进行酶切。分子量标准:Lambda-HindIII。 The 3 tracking dyes enable easy optimization of gel electrophoresis times and monitoring of small DNA fragments. 微量离心。 QIAcube. greenwich pottery houseWebb15 maj 2024 · 加入转染试剂到每个孔的培养基中,6h后,更换成完全培养基,继续培养到24-48小时 (不同的质粒和脂质体最佳搭配比例不同,转染前,应该摸一个最佳配比。. 质 … greenwich presbyterian church cemeteryWebb31 maj 2024 · 荧光素酶报告基因实验(luciferase assay)是检测这类转录因子和其靶启动子中的特异顺序结合的重要手段。. 其原理简述如下:. (1)构建一个将靶启动子的特定 … foam covers for coat hangersWebbVector Name: pHIS8 Source: Center for Structures of Membrane Proteins Description: Gateway compatible bacterial expression vector with T7 promoter, N- and C-terminal His tags and thrombin cleavage site; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning. … foam covers for headset earpieceWebb28 sep. 2024 · 解剖式学习一个质粒结构--update. 半年后阅读量已经达到6700,因此特别担心自己的初级之作没有起到很好的帮助,对读者造成误解,因此在这里增加一个新的版 … foam covers for beds