Svct2 western blot

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August undefined, 2024

SpletTecnólogo Médico en Morfofisiopatología y Citodiagnóstico, además cursé 4 años de Doctorado en Ciencias Biológicas, área Biología Celular y Molecular. Experiencia en diversas técnicas de laboratorio: PCR, Inmunohistoquímica, Inmunofluorescencia, Western Blot, Histología, Cultivo celular primario y de líneas celulares, Microscopía, entre otras. … SpletConceptualized a project on autophagy, by performing lentiviral transduction, stable cell line generation, transient transfection, Western blot, protein-protein interaction, immunofluorescence, cloning, site directed mutagenesis and chromatin immunoprecipitation, resulting in understanding the mechanism of action (MOA) of …

Sodium-dependent vitamin C transporter 2 (SVCT2) expression …

SpletFP-translation of SCO-spondin was demonstrated by western blot analysis and immunocytochemistry. These results support previous evidence ascribing CNS developmental properties to SCO-spondin. ... two different isoforms of sodium–vitamin C cotransporters (SVCT1/SLC23A1 and SVCT2/SLC23A2) have been cloned. SVCT2 was … SpletLa technique de Western Blot est composée de 4 étapes : - La séparation des protéines par électrophorèse sur gel. - Le transfert des protéines sur un support solide. - La détection de la protéine cible. - La visualisation de la protéine cible. Après l'étape de transfert, la membrane subit une étape de blocage qui recouvre toute la ... flashpoint trailer

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Splet11. feb. 2011 · To assess the levels of SVCT2 on a whole-protein level, brains of stroke mice were dissected and lysed at day 0, day 2 and day 5 after stroke and analysed by western … Splet26. maj 2024 · Test Western Blot znajduje zastosowanie w diagnostyce chorób wywoływanych przez wirusy jako metoda wykrywania ich rdzeni. Najczęściej wykorzystywany jest przy diagnozowaniu wirusowego zapalenia wątroby typu C, opryszczki, SARS, a po uprzednim wykonaniu testu ELISA również zakażenia wirusem HIV. Test … Splet1. Western blotting 원리. Western blot은 세포나 조직에서 얻은 단백질들에서 특정 단백질 (specific protein)을 검출할 수 있는 실험 방법이다. 기본 원리는 단백질을 size별로 분류한 뒤에 발현 양을 확인하고 싶은 항체를 이용하여 확인 하는 것이다. 즉, 항원-항체 반응을 ... flashpoint candle making

Introducción a Western Blot - AllScience

Anti-SLC23A2 / SVCT2 Antibody Rabbit anti-Human Polyclonal …

Splet12. feb. 2024 · SVCT2, Sodium-dependent Vitamin C Transporter 2, uniquely transports ascorbic acid (also known as vitamin C and ascorbate) into all types of cells. Vitamin C is … SpletAlomone Labs, LTD Anti-SVCT2/SLC23A2 (extracellular) Polyclonal, Catalog # AST-022-200UL. Tested in Western Blot (WB), Immunohistochemistry (Frozen) (IHC (F)) and Flow … flashpacker companies houseSpletWestern Blot: Human: Lee SH, Kim JS, Jun HK The major outer membrane protein of a periodontopathogen induces IFN-beta and IFN-stimulated genes in monocytes via lipid raft and TANK-binding kinase 1/IFN regulatory factor-3. J. Immunol., 2009;182(9):5823-35. 2009 [PMID: 19380831] (Western Blot, Human) flask backend local testing

"SpletSVCT2 antibody LS-C334917 is an unconjugated rabbit polyclonal antibody to SVCT2 (SLC23A2) from human. It is reactive with human, mouse and rat. Validated for WB. … " - Svct2 western blot

Svct2 western blot

Western blot – Wikipédia, a enciclopédia livre

Splet01. sep. 2024 · Western blot analyses were performed using antibodies against actin, SVCT2, cytochrome c, Hsp-60, calnexin and GLUT3. Details on the Western blotting … Splet11. feb. 2011 · Time-course analyses of SVCT2 expression by immunohistochemistry and western blots showed upregulation in the subacute phase of 2-5 days. Radioactive uptake assays using (14)C-labelled ascorbic acid showed a significant increase of ascorbic acid uptake into the brain after stroke.

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SpletApplications: Western blot SLC23A2 / SVCT2 Antibody (aa251‑300, Biotin) LS‑C445773 Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, … SpletAnti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western …

SpletBackground and objective. Activation and abnormal expression of histone deacetylase (HDAC) which is important target for cancer therapeutics are related to the occurrence of human leukemia. 20(s)-Ginsenoside Rh2 (20(s)-Rh2) may be a potential HDAC inhibitor (HDACi) of leukemia, but the mechanism has not been reported. SpletSVCT2 staining (green) in CA1 hippocampal region is detected in axonal processes (arrows), with the highest density surrounding the pyramidal layer (P). Cell nuclei are stained... View more Product Details Product Specific Information Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size.

Splet14. feb. 2024 · The process of obtaining ascorbic acid (AA) via intestinal absorption and blood circulation is carrier-mediated utilizing the AA transporters SVCT1 and SVCT2, which are expressed in the intestine and brain (SVCT2 in abundance). SpletSVCT2 is a reported alias name for the human gene SLC23A2, or 'solute carrier family 23 member 2'. The 650-amino acid protein is a member of the Xanthine/uracil permease family, Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. The cellular localization is predicted to be membrane-associated. Glycosylation sites have been reported.

SpletThis gene encodes one of the two required transporters and the encoded protein accounts for tissue-specific uptake of vitamin C. Previously, this gene had an official symbol of SLC23A1.The absorption of vitamin C into the body and its distribution to organs requires two sodium-dependent vitamin C transporters.

Splet( E) Western blots for SVCT2 in biotinylated fractions (membrane) or whole lysates (input) from intact mouse retinas ( n = 2 animals) or N9 microglia ( n = 3 independent cultures). ( … flat 1940s womens shoesSplet(c) Western blot analysis of U-U937 and D-U937 cell-derived mitochondrial lysates (10 and 15 μ g of mitochondrial proteins) using anti-SVCT2 antibodies. Anti-HSP-60 antibodies were used to provide an internal loading control and for comparative densitometric analysis. flat backed bathtub matsSpletWestern blot，中文为蛋白质免疫印迹试验，简单来说是抗原抗体特异性结合。. 电泳分离后的蛋白转移到固相载体PVDF膜上，以共价键的形式吸附蛋白质，并作为抗原结合特异性抗体。. 二抗作为显色标记，经过底物显色反应组织和细胞中蛋白的表达情况。. flask cross site scriptingSpletAnti-SVCT2 Western Blot Antibody Products. Products (26) Write a Review; Company View; Product View; Your search returned 26 SVCT2 Western Blot Antibodies across 5 suppliers. Showing 5 of 5 suppliers (26 products total) > >> Select All. Select up to 5 products from below to compare or request more information. flask template for eachSplet23. nov. 2011 · Two days after transfection, Schwann cells were harvested for Western blotting or real-time PCR. SVCT2 siRNA-treated Schwann cell cultures showed a significant reduction in collagen V chains (Fig. 7B). Western blot bands of SVCT2 showed weaker bands in siRNA-treated Schwann cell cultures, confirming successful knockdown (Fig. 7B). flat backingSpletWestern blot mitjançant un anticòs que reconeix proteïnes modificades amb àcid lipoic. El western blot (de vegades anomenat proteïna immunoblot), o western blot, és una tècnica analítica àmpliament utilitzada en biologia molecular i immunogenètica per a detectar proteïnes específiques en una mostra d'homogeneïtzació o extracte de teixits.. En resum, … flask shaped meaningSplet28. jun. 2024 · Western Blot es una técnica analítica, ampliamente utilizada, para el estudio de proteínas. Este método permite la detección de una sola proteína dentro de una muestra biológica. La especificidad del Western Blot se logra mediante el uso de un anticuerpo que reconoce y se une a un epítopo único de la proteína de interés. flashshop.cc